Application
Fig1: Western blot analysis of Matrin 3 on different lysates using anti-Matrin 3 antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: Mouse brain tissueApplication
Fig2: ICC staining Matrin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Matrin 3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Matrin 3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of LOVO cells with Matrin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Matrin 3 [JU93-43] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALS21 antibody KIAA0723 antibody Matr3 antibody MATR3_HUMAN antibody Matrin-3 antibody Matrin3 antibody MPD2 antibody VCPDM antibody Vocal cord and pharyngeal weakness with distal myopathy antibody |
| Product description | Matrin-3 is a nuclear matrix protein containing one matrin-type zinc finger and two RRM (RNA recognition motif) domains. Matrin-3 plays a role in transcription and may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the PSF-p54/NRB heteromer, Matrin-3 may play a role in the nuclear retention of defective RNAs. As the main substrate for PKA-mediated phosphorylation, Matrin-3 may serve as a rapid way of transferring information from synapses containing NMDA receptors to neuronal nuclei under physiological conditions. Also, the phosphorylation of Matrin-3 may contribute to neuronal death under pathological conditions. It is likely that Matrin-3 activity is regulated by calcium dependent interaction with CaM I and also by caspase induced cleavage. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ALS21 antibody KIAA0723 antibody Matr3 antibody MATR3_HUMAN antibody Matrin-3 antibody Matrin3 antibody MPD2 antibody VCPDM antibody Vocal cord and pharyngeal weakness with distal myopathy antibody |
|---|---|
| Molecular Weight(MW) | 95 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Matrin 3 on different lysates using anti-Matrin 3 antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: Mouse brain tissue
Application
Fig2: ICC staining Matrin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Matrin 3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Matrin 3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of LOVO cells with Matrin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Daudi, mouse brain tissue lysate, Hela, LOVO, SH-SY-5Y, rat brain tissue, human tonsil tissue, human lung cancer tissue, human liver tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
