WB
Western blot analysis of SFPQ on different lysates with Rabbit anti-SFPQ antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane), Lane 2: 293T cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (20 µg/Lane), Lane 5: Daudi cell lysate (20 µg/Lane), Lane 6: Neuro-2a cell lysate (20 µg/Lane), Lane 7: NIH/3T3 cell lysate (20 µg/Lane), Lane 8: C2C12 cell lysate (20 µg/Lane), Lane 9: C6 cell lysate (20 µg/Lane), Lane 10: Mouse testis tissue lysate (40 µg/Lane), Lane 11: Mouse lung tissue lysate (40 µg/Lane), Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SFPQ antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SFPQ antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SFPQ antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SFPQ antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SFPQ antibody. Counter stained with hematoxylin.ICC/IF
Immunocytochemistry analysis of Daudi cells labeling SFPQ with Rabbit anti-SFPQ antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SFPQ antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | SFPQ Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. |
| Immunogen | Synthetic peptide within Human SFPQ aa 658-707 / 707. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:1000 ICC/IF:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 100 kDa DNA pairing protein antibody 100 kDa DNA-pairing protein antibody 100 kDa subunit antibody DNA binding p52/p100 complex 100 kDa subunit antibody DNA-binding p52/p100 complex antibody hPOMp100 antibody Polypyrimidine tract binding protein associated splicing factor antibody Polypyrimidine tract-binding protein-associated-splicing factor antibody POMP100 antibody PPP1R140 antibody proline- and glutamine-rich antibody Protein phosphatase 1 regulatory subunit 140 antibody PSF antibody PTB associated splicing factor antibody PTB-associated-splicing factor antibody Sfpq antibody SFPQ_HUMAN antibody Splicing factor antibody Splicing factor proline and glutamine rich antibody Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody Splicing factor proline/glutamine rich antibody |
|---|---|
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
WB
Western blot analysis of SFPQ on different lysates with Rabbit anti-SFPQ antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane), Lane 2: 293T cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (20 µg/Lane), Lane 5: Daudi cell lysate (20 µg/Lane), Lane 6: Neuro-2a cell lysate (20 µg/Lane), Lane 7: NIH/3T3 cell lysate (20 µg/Lane), Lane 8: C2C12 cell lysate (20 µg/Lane), Lane 9: C6 cell lysate (20 µg/Lane), Lane 10: Mouse testis tissue lysate (40 µg/Lane), Lane 11: Mouse lung tissue lysate (40 µg/Lane), Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SFPQ antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
ICC/IF
Immunocytochemistry analysis of Daudi cells labeling SFPQ with Rabbit anti-SFPQ antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SFPQ antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:500-1:2000 IHC:1:1000 ICC/IF:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
