Application
Fig1: Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution. Positive control: Lane 1: Mouse thymus tissue Lane 2: SiHaApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM5 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM5 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCM5 antibody. Counter stained with hematoxylin.| Product Name | MCM5 [JB31-34] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC 46 antibody CDC46 antibody CDC46 homolog antibody Cell division cycle 46 antibody DNA replication licensing factor antibody DNA replication licensing factor MCM 5 antibody DNA replication licensing factor MCM5 antibody MCM 5 antibody MCM5 antibody MCM5_HUMAN antibody MGC5315 antibody Minichromosome maintenance complex component 5 antibody Minichromosome maintenance deficient (S. cerevisiae) 5 antibody Minichromosome maintenance deficient 5 antibody Minichromosome maintenance deficient protein 5 antibody P1 CDC46 antibody P1-CDC46 antibody |
| Product description | The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. |
| Immunogen | Recombinant protein within C terminal human MCM5. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:1,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CDC 46 antibody CDC46 antibody CDC46 homolog antibody Cell division cycle 46 antibody DNA replication licensing factor antibody DNA replication licensing factor MCM 5 antibody DNA replication licensing factor MCM5 antibody MCM 5 antibody MCM5 antibody MCM5_HUMAN antibody MGC5315 antibody Minichromosome maintenance complex component 5 antibody Minichromosome maintenance deficient (S. cerevisiae) 5 antibody Minichromosome maintenance deficient 5 antibody Minichromosome maintenance deficient protein 5 antibody P1 CDC46 antibody P1-CDC46 antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Nucleus. Cytosol. |
Application
Fig1: Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution. Positive control: Lane 1: Mouse thymus tissue Lane 2: SiHa
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCM5 antibody. Counter stained with hematoxylin.| Positive Control | Mouse thymus tissue lysate, SiHa, human tonsil tissue, human placenta tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
