Application
Fig1: Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.Application
Fig2: ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Wnt2b [JB48-34] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein Wnt-13 antibody Protein Wnt-2b antibody Wingless type MMTV integration site family, member 13 antibody Wingless type MMTV integration site family, member 2B antibody WNT13 antibody Wnt2b antibody WNT2B_HUMAN antibody XWNT2 antibody XWNT2, Xenopus, homolog of antibody |
| Product description | This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCICCIFFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Protein Wnt-13 antibody
Protein Wnt-2b antibody
Wingless type MMTV integration site family member 13 antibody Wingless type MMTV integration site family member 2B antibody WNT13 antibody Wnt2b antibody WNT2B_HUMAN antibody XWNT2 antibody XWNT2 Xenopus homolog of antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Secreted. Extracellular region. |
Application
Fig1: Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.
Application
Fig2: ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SiHa, Hela, LOVO, SHSY-5Y, human kidney tissue, mouse kidney tissue, rat testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
