WB
Western blot analysis of Furin on different lysates with Rabbit anti-Furin antibody at 1/2,000 dilution. Lane 1: 293T cell lysate, Lane 2: A431 cell lysate, Lane 3: Ramos cell lysate (low expression), Lane 4: C2C12 cell lysate, Lane 5: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Furin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Furin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Furin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Furin antibody. Counter stained with hematoxylin.| Product Name | Furin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Furin (FUR, PACE, PCSK3, SPC1, Kex2p) is a calcium-dependent serine endoprotease that belongs to the subtilisin-like proprotein convertase family. The members of this family process latent precursor proteins into their biologically active products. Furin cleaves at paired basic amino acid processing sites within proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. Furin can directly cleave proMMP-2 within the trans-Golgi network leading to an inactive form of matrix metalloproteinase-2 (MMP-2). Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The inhibitory mechanism is based on the presence of an inactivating prosegment at the NH2 terminal of the Furin. After initial autocatalytic cleavage, the prosegment remains tightly associated until it reaches the trans-Golgi network where the dissociation of the prosegment and activation of furin occurs. |
| Immunogen | Recombinant protein within Human Furin aa 261-310 / 794. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Dibasic processing enzyme antibody Dibasic-processing enzyme antibody FES upstream region antibody FUR antibody FURIN antibody Furin membrane associated receptor protein antibody FURIN_HUMAN antibody PACE antibody Paired basic amino acid residue cleaving enzyme antibody Paired basic amino acid residue-cleaving enzyme antibody PCSK3 antibody Proprotein convertase subtilisin/kexin type 3 antibody SPC1 antibody |
|---|---|
| Molecular Weight(MW) | 87 kDa(Observed band size: 75 kDa) |
| Cellular Localization | Secreted. Plasma membrane. Golgi apparatus. Endosome. |
| SwissProt ID | P09958 |
|---|
WB
Western blot analysis of Furin on different lysates with Rabbit anti-Furin antibody at 1/2,000 dilution. Lane 1: 293T cell lysate, Lane 2: A431 cell lysate, Lane 3: Ramos cell lysate (low expression), Lane 4: C2C12 cell lysate, Lane 5: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Furin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Furin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Furin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Furin antibody. Counter stained with hematoxylin.| Application Notes | WB:1:2000 IHC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
