Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | KDM5A [JB36-60] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone demethylase JARID1A antibody JARID1A antibody Jumonji/ARID domain containing protein 1A antibody Jumonji/ARID domain-containing protein 1A antibody Kdm5a antibody KDM5A_HUMAN antibody Lysine-specific demethylase 5A antibody RBBP-2 antibody RBBP2 antibody RBP2 antibody Retinoblastoma binding protein 2 antibody Retinoblastoma-binding protein 2 antibody |
| Product description | Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1. Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFFC |
| WB:1:500 ICC:1:50-1:400 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Histone demethylase JARID1A antibody JARID1A antibody Jumonji/ARID domain containing protein 1A antibody Jumonji/ARID domain-containing protein 1A antibody Kdm5a antibody KDM5A_HUMAN antibody Lysine-specific demethylase 5A antibody RBBP-2 antibody RBBP2 antibody RBP2 antibody Retinoblastoma binding protein 2 antibody Retinoblastoma-binding protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 192 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P29375 |
|---|
Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, SiHa, SH-SY-5Y. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:400 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
