Application
Fig1: Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.Application
Fig2: ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | RPA70 [JB75-32] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dmrpa1 antibody Drosophila Replication Protein A antibody DRPA antibody HSSB antibody Human single stranded DNA binding protein antibody MST075 antibody MSTP075 antibody p70 antibody REPA1 antibody Replication factor A antibody Replication factor A protein 1 antibody Replication protein A 70 kDa DNA-binding subunit antibody Replication protein A 70kDa DNA binding subunit antibody Replication protein A1 70kDa antibody Replication protein A1 antibody RF A antibody RF-A protein 1 antibody RFA antibody RFA1_HUMAN antibody RP A antibody RP-A p70 antibody RPA 70 antibody RPA antibody rpa1 antibody Single stranded binding protein 70 antibody Single-stranded DNA-binding protein antibody |
| Product description | The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner. |
| Immunogen | Recombinant protein corresponding to Human RPA70 aa 1-100. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFFC |
| WB:1:500 ICC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Dmrpa1 antibody Drosophila Replication Protein A antibody DRPA antibody HSSB antibody Human single stranded DNA binding protein antibody MST075 antibody MSTP075 antibody p70 antibody REPA1 antibody Replication factor A antibody Replication factor A protein 1 antibody Replication protein A 70 kDa DNA-binding subunit antibody Replication protein A 70kDa DNA binding subunit antibody Replication protein A1 70kDa antibody Replication protein A1 antibody RF A antibody RF-A protein 1 antibody RFA antibody RFA1_HUMAN antibody RP A antibody RP-A p70 antibody RPA 70 antibody RPA antibody rpa1 antibody Single stranded binding protein 70 antibody Single-stranded DNA-binding protein antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P27694 |
|---|
Application
Fig1: Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution.
Application
Fig2: ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Siha, 293T, A549, LOVO. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
