Application
Fig1: Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1,000 dilution. Positive control： Lane 1: Rat testis tissue Lane 2: Human placenta tissue Lane 3: Mouse testis tissue Lane 4: 293Application
Fig2: ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining KDEL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining KDEL in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KDEL antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-KDEL antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-KDEL antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDEL antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | KDEL [JB42-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ER lumen protein retaining receptor 1 antibody ERD2.1 antibody ERD21_HUMAN antibody KDEL endoplasmic reticulum protein retention receptor 1 antibody KDEL receptor 1 antibody Kdelr1 antibody Putative MAPK-activating protein PM23 antibody |
| Product description | Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrograde vesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus. When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor. Subsequently, this interaction allows the KDEL receptor to recruit a GTPase-activating protein (GAP) from the cytosol to membranes, which inactivates ARF1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:2000 ICC:1:400-1:800 IHC:1:100-1:400 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ER lumen protein retaining receptor 1 antibody ERD2.1 antibody ERD21_HUMAN antibody KDEL endoplasmic reticulum protein retention receptor 1 antibody KDEL receptor 1 antibody Kdelr1 antibody Putative MAPK-activating protein PM23 antibody |
|---|---|
| Molecular Weight(MW) | 24/17 kDa(Predicted band size) |
| Cellular Localization | Endoplasmic reticulum. Membrane. |
Application
Fig1: Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1,000 dilution. Positive control： Lane 1: Rat testis tissue Lane 2: Human placenta tissue Lane 3: Mouse testis tissue Lane 4: 293
Application
Fig2: ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining KDEL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining KDEL in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KDEL antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-KDEL antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-KDEL antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDEL antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Rat testis tissue, Mouse testis tissue, 293, HepG2, A549, 293T, rat epididymis tissue, human placenta tissue, human stomach cancer tissue, mouse small intestine tissue. |
|---|---|
| Application Notes | WB:1:500-1:2000 ICC:1:400-1:800 IHC:1:100-1:400 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
