WB
Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Thrombomodulin antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Thrombomodulin antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Thrombomodulin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Thrombomodulin (TM), also called CD141, is a type I membrane receptor that is specific to endothelial cells. TM has a cysteine-rich extracellular domain with six EGF-like regions. It forms a complex with Thrombin, which activates Protein C to generate activated Protein C (APC), an anticoagulant enzyme. APC together with Protein S inhibits coagulation by inactivating Factors Va and VIIIa. Deletion of the TM gene results in embryonic lethality in mice. |
| Immunogen | Synthetic peptide within Human Thrombomodulin aa 526-575 / 575. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | AHUS 6 antibody AHUS6 antibody BDCA 3 antibody BDCA3 antibody CD 141 antibody CD141 antibody CD141 antigen antibody Fetomodulin antibody Thbd antibody THPH12 antibody THRM antibody Thrombomodulin antibody TM antibody TRBM_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P07204 |
|---|
WB
Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Thrombomodulin antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Thrombomodulin antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IP:1:10-1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
