Application
Fig1: Western blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution.Application
Fig2: ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CAMKIV in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CAMKIV in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with CAMKIV antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CAMKIV [JB33-13] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody Brain Ca(2+) calmodulin dependent protein kinase type IV antibody Brain Ca++-calmodulin dependent protein kinase type IV antibody Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody Calcium/calmodulin dependent protein kinase IV antibody Calcium/calmodulin dependent protein kinase type IV antibody Calcium/calmodulin-dependent protein kinase type IV antibody CAM kinase 4 antibody CAM kinase GR antibody CAM kinase IV antibody CAM kinase-GR antibody CaMK 4 antibody CAMK GR antibody CaMK IV antibody Camk4 antibody CaMKGR antibody IV antibody KCC4_HUMAN antibody MGC36771 antibody |
| Product description | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Immunogen | Recombinant protein corresponding to the C-terminus of human CAMKIV. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody Brain Ca(2+) calmodulin dependent protein kinase type IV antibody Brain Ca++-calmodulin dependent protein kinase type IV antibody Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody Calcium/calmodulin dependent protein kinase IV antibody Calcium/calmodulin dependent protein kinase type IV antibody Calcium/calmodulin-dependent protein kinase type IV antibody CAM kinase 4 antibody CAM kinase GR antibody CAM kinase IV antibody CAM kinase-GR antibody CaMK 4 antibody CAMK GR antibody CaMK IV antibody Camk4 antibody CaMKGR antibody IV antibody KCC4_HUMAN antibody MGC36771 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CAMKIV in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CAMKIV in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with CAMKIV antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | 293T, Hela, SH-SY-5Y, rat brain tissue, human tonsil tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
