WB
Western blot analysis of CENPC on different lysates with Rabbit anti-CENPC antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane), Lane 2: HL-60 cell lysate (20 µg/Lane), Lane 3: NIH/3T3 cell lysate (20 µg/Lane), Lane 4: C2C12 cell lysate (20 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CENPC antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CENPC antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | CENPC Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle. |
| Immunogen | Recombinant protein within Human CENPC aa 360-540 / 943. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CENP-C 1 antibody CENP-C antibody CENPC_HUMAN antibody CENPC1 antibody Centromere autoantigen C antibody Centromere autoantigen C1 antibody Centromere protein C 1 antibody Centromere protein C antibody hcp-4 antibody ICEN7 antibody Interphase centromere complex protein 7 antibody MIF2 antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q03188 |
|---|
WB
Western blot analysis of CENPC on different lysates with Rabbit anti-CENPC antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane), Lane 2: HL-60 cell lysate (20 µg/Lane), Lane 3: NIH/3T3 cell lysate (20 µg/Lane), Lane 4: C2C12 cell lysate (20 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CENPC antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CENPC antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
