Application
Fig1: Western blot analysis of Cellubrevin on A549 (1), 293T(2) cell lysates using anti-Cellubrevin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cellubrevin in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Cellubrevin [JG36-31] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CEB antibody Cellubrevin antibody Synaptobrevin 3 antibody Synaptobrevin-3 antibody VAMP 3 antibody VAMP-3 antibody VAMP3 antibody VAMP3_HUMAN antibody Vesicle associated membrane protein 3 antibody Vesicle-associated membrane protein 3 antibody |
| Product description | Vesicle-associated membrane proteins, known as VAMPs, also designated synaptobrevins, include VAMP-1, VAMP-2, VAMP-3 (cellubrevin), and synaptotagmin, a protein that may function as an inhibitor of exocytosis. VAMP proteins are vesicular factors that are important components of the machinery controlling docking and/or fusion of secretory vesicles with their target membrane. Synaptosomal-associated proteins, known as SNAPs, including alpha- and gamma-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. Pancreatic beta-cells express VAMP-2 and VAMP-3, and either one or both of these proteins selectively control Ca2+-mediated insulin secretion. In addition, VAMP-2 and VAMP-3 are expressed on GLUT4-containing vesicle membranes isolated from 3T3-Ll adipocytes and are important components of the insulin-dependent translocation of GLUT4 to the cell surface in adipocytes. |
| Immunogen | Synthetic peptide conjugated to KLH within N-terminal human Cellubrevin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CEB antibody Cellubrevin antibody Synaptobrevin 3 antibody Synaptobrevin-3 antibody VAMP 3 antibody VAMP-3 antibody VAMP3 antibody VAMP3_HUMAN antibody Vesicle associated membrane protein 3 antibody Vesicle-associated membrane protein 3 antibody |
|---|---|
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of Cellubrevin on A549 (1), 293T(2) cell lysates using anti-Cellubrevin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cellubrevin in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | 293T, SiHa, rat brain tissue, rat hippocampus tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
