WB
Western blot analysis of Mib1 on different lysates with Rabbit anti-Mib1 antibody at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane), Lane 2: U-87MG cell lysate (20 µg/Lane), Lane 3: Mouse brain tissue lysate (40 µg/Lane), Lane 4: Mouse testis tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining Mib1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Mib1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Mib1 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Mib1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Mib1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. |
| Immunogen | Recombinant protein within Human Mib1 aa 2-93 / 1006. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | DAPK-interacting protein 1 antibody Dip 1 antibody DIP-1 antibody Dip1 antibody E3 ubiquitin protein ligase MIB 1 antibody E3 ubiquitin protein ligase MIB1 antibody E3 ubiquitin-protein ligase mib1 antibody KIAA1323 antibody LVNC7 antibody MIB antibody mib1 antibody MIB1_HUMAN antibody Mind bomb homolog 1 antibody Mindbomb E3 ubiquitin protein ligase 1 antibody Ubiquitin ligase mind bomb antibody Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody ZZANK2 antibody ZZZ6 antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Plasma membrane. Cytoplasm. Cytoskeleton. |
| SwissProt ID | Q86YT6 |
|---|
WB
Western blot analysis of Mib1 on different lysates with Rabbit anti-Mib1 antibody at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane), Lane 2: U-87MG cell lysate (20 µg/Lane), Lane 3: Mouse brain tissue lysate (40 µg/Lane), Lane 4: Mouse testis tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining Mib1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Mib1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Mib1 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Mib1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
