Application
Fig1: Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1,000 dilution.Application
Fig2: ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | PIST [JG79-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAL antibody CFTR associated ligand antibody CFTR-associated ligand antibody dJ94G16.2 antibody dJ94G16.2 PIST antibody FIG antibody Fused in glioblastoma antibody Golgi associated PDZ and coiled coil motif containing antibody Golgi associated PDZ and coiled coil motif containing protein antibody Golgi-associated PDZ and coiled-coil motif-containing protein antibody GOPC 1 antibody GOPC antibody GOPC_HUMAN antibody GOPC1 antibody OTTHUMP00000040403 antibody PDZ protein interacting specifically with TC 10 antibody PDZ protein interacting specifically with TC10 antibody PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody PIST antibody Protein interacting specifically with Tc 10 antibody Protein interacting specifically with Tc10 antibody |
| Product description | PIST (PDZ protein interacting specifically with TC10), also known as GOPC (golgi associated PDZ and coiled-coil motif containing), CAL or FIG, is a 462 amino acid protein that localizes to the cytoplasm, as well as to the membrane of the golgi apparatus and to the cell junction. Expressed ubiquitously and containing one PDZ (DHR) domain, PIST functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, PIST is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding PIST are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Three isoforms of PIST exist due to alternative splicing events. |
| Immunogen | Recombinant protein within human PIST aa 1-100. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIPIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CAL antibody CFTR associated ligand antibody CFTR-associated ligand antibody dJ94G16.2 antibody dJ94G16.2 PIST antibody FIG antibody Fused in glioblastoma antibody Golgi associated PDZ and coiled coil motif containing antibody Golgi associated PDZ and coiled coil motif containing protein antibody Golgi-associated PDZ and coiled-coil motif-containing protein antibody GOPC 1 antibody GOPC antibody GOPC_HUMAN antibody GOPC1 antibody OTTHUMP00000040403 antibody PDZ protein interacting specifically with TC 10 antibody PDZ protein interacting specifically with TC10 antibody PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody PIST antibody Protein interacting specifically with Tc 10 antibody Protein interacting specifically with Tc10 antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm. Golgi apparatus. Plasma membrane. |
Application
Fig1: Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1,000 dilution.
Application
Fig2: ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SiHa, HepG2, MCF-7, SKOV-3, human liver cancer tissue, human colon tissue, human kidney tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
