Application
Fig1: Western blot analysis of Tropomyosin 1 on rat heart tissue using anti-Tropomyosin 1 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of MCF-7 cells with Tropomyosin 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Tropomyosin 1 [JG38-66] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin α is encoded by the TPM1 gene, which maps to human chromosome 15q22.1 and undergoes alternative splicing to generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Tropomyosin β is encoded by the TPM2 gene, which maps to human chromosome 9p13.3 and undergoes alternative splicing to generate three isoforms, including skeletal muscle (isoform 1), non-muscle/fibroblast TM36/epithelial TMe1 (isoform 2) and non-muscle (isoform 3). Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction. |
| Immunogen | Recombinant protein within human Tropomyosin 1 aa 100-300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,IHC,FC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | AA986836 antibody
AI854628 antibody
Alpha tropomyosin antibody
alpha-TM antibody
Alpha-tropomyosin antibody
C15orf13 antibody
cardiomyopathy hypertrophic 3 antibody CMD1Y antibody CMH3 antibody HTM alpha antibody HTM-alpha antibody OTTHUMP00000163688 antibody sarcomeric tropomyosin kappa antibody TM2 antibody Tmpa antibody TMSA antibody Tpm-1 antibody TPM1 antibody TPM1_HUMAN antibody tropomyosin 1 (alpha) antibody tropomyosin 1 (alpha) isoform 1 antibody tropomyosin 1 (alpha) isoform 2 antibody tropomyosin 1 (alpha) isoform 3 antibody tropomyosin 1 (alpha) isoform 4 antibody tropomyosin 1 (alpha) isoform 5 antibody tropomyosin 1 (alpha) isoform 6 antibody tropomyosin 1 (alpha) isoform 7 antibody Tropomyosin 1 antibody Tropomyosin alpha 1 chain antibody Tropomyosin alpha-1 chain antibody Tropomyosin skeletal muscle alpha antibody Tropomyosin-1 antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Cytoskeleton. |
Application
Fig1: Western blot analysis of Tropomyosin 1 on rat heart tissue using anti-Tropomyosin 1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of MCF-7 cells with Tropomyosin 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat heart tissue, rat smooth muscle tissue, human heart tissue, mouse skeletal muscle tissue, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
