Application
Fig1: Western blot analysis of COMT on MCF-7 cell (1) and rat testis tissue (2) lysate using anti-COMT antibody at 1/2,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-COMT antibody. Counter stained with hematoxylin.| Product Name | COMT [JG39-48] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Catechol O methyltransferase antibody Catechol O-methyltransferase antibody COMT antibody COMT_HUMAN antibody EC 2.1.1.6 antibody |
| Product description | Catechol-O-methyltransferase (COMT) plays a crucial role in the regulation of central dopaminergic systems by catalyzing the inactivation of catechol-amines. It is widely distributed in most tissues in soluble and membrane-bound forms. COMT-mediated methylation metabolism of catecholamine neurotransmitters is a first-line detoxification pathway. A Val158Met polymorphism of the COMT gene affects activity of the enzyme and influences performance and efficiency of the prefrontal cortex of the brain. Sequential conversion of estradiol to methoxyestradiol by COMT, contributes to the antimitogenic effects of estradiol on vascular smooth muscle cell growth via estrogen receptor-independent mechanisms. |
| Immunogen | Recombinant protein of C-terminal human COMT. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Catechol O methyltransferase antibody Catechol O-methyltransferase antibody COMT antibody COMT_HUMAN antibody EC 2.1.1.6 antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Cell membrane. Cytoplasm. |
Application
Fig1: Western blot analysis of COMT on MCF-7 cell (1) and rat testis tissue (2) lysate using anti-COMT antibody at 1/2,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-COMT antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, rat testis tissue lysate, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
