WB
Western blot analysis of FKBP52 on different lysates with Rabbit anti-FKBP52 antibody at 1/500 dilution. Lane 1: SK-Br-3 cell lysate, Lane 2: Hela cell lysate, Lane 3: M.testis lysate, Lane 4: R.testis lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining FKBP52 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-FKBP52 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of 293T cells labeling FKBP52. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | FKBP52 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria. |
| Immunogen | Recombinant protein within Human FKBP52 aa 1-132 / 459. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:1000 ICC/IF:1:50-1:200 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 51 kDa FK506-binding protein antibody 52 kDa FK506 binding protein antibody 52 kDa FK506-binding protein antibody 52 kDa FKBP antibody 59 kDa immunophilin antibody FK506 binding protein 4 antibody FK506-binding protein 4 antibody FKBP 4 antibody FKBP 52 antibody FKBP 59 antibody FKBP-4 antibody FKBP-52 antibody FKBP4 antibody FKBP4_HUMAN antibody FKBP51 antibody FKBP52 protein antibody FKBP59 antibody HBI antibody Hsp 56 antibody HSP binding immunophilin antibody HSP-binding immunophilin antibody Hsp56 antibody Immunophilin FKBP52 antibody N-terminally processed antibody p52 antibody p59 antibody p59 protein antibody Peptidyl prolyl cis trans isomerase antibody Peptidyl-prolyl cis-trans isomerase FKBP4 antibody Peptidylprolyl cis trans isomerase antibody PPIase antibody PPIase FKBP4 antibody Rotamase antibody T cell FK506 binding protein 59kD antibody |
|---|---|
| Molecular Weight(MW) | 52kDa(Observed band size: 55kDa) |
| Cellular Localization | Cytoplasm. Cytoskeleton. Microtubule. Mitochondrion. Nucleus. |
| SwissProt ID | Q02790 |
|---|
WB
Western blot analysis of FKBP52 on different lysates with Rabbit anti-FKBP52 antibody at 1/500 dilution. Lane 1: SK-Br-3 cell lysate, Lane 2: Hela cell lysate, Lane 3: M.testis lysate, Lane 4: R.testis lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining FKBP52 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-FKBP52 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of 293T cells labeling FKBP52. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000-1:2000 IHC:1:1000 ICC/IF:1:50-1:200 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
