Application
Fig1: Western blot analysis of Syntenin on A549 cell using anti-Syntenin antibody at 1/2,000 dilution.Application
Fig2: ICC staining Syntenin in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Syntenin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syntenin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntenin antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of A549 cells with Syntenin antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Syntenin [JE40-72] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MDA-9 antibody MDA9 antibody Melanoma differentiation-associated protein 9 antibody Pro-TGF-alpha cytoplasmic domain-interacting protein 18 antibody Scaffold protein Pbp1 antibody SDCB1_HUMAN antibody SDCBP antibody ST1 antibody SYCL antibody Syndecan binding protein (syntenin) antibody Syndecan binding protein 1 antibody Syndecan-binding protein 1 antibody Syntenin 1 antibody Syntenin-1 antibody TACIP18 antibody |
| Product description | Syntenin-1 (also known as syntenin, syndecan binding protein, melanoma differentiation-associated protein 9 or proTGF-alpha cytoplasmic domain-interacting protein 18) is a protein that binds to the cytoplasmic domains of the syndecans in yeast 2-hybrid screens and other assays. Syntenin-1 contains a tandem repeat of PDZ domains that reacts with the FYA (phe-tyr-ala) carboxy-terminal amino acid sequence of the syndecans. It may function as an adaptor that couples syndecans to cytoskeletal proteins or cytosolic downstream signal-effectors. Syntenin-1 colocalizes and interacts specifically with immature, intracellular forms of proTGF-alpha. It is a human gamma interferon responsive protein. Syntenin-1 contains PSD-95/Discs large/zO-1 (PDZ) domains and associates with the cytoplasmic tail of the IL-5Ralpha. It directly associates with the transcription factor Sox-4. The PDZ proteins PICK1, GRIP, ABP and syntenin-1 bind multiple glutamate receptor subtypes. |
| Immunogen | Recombinant protein within human Syntenin aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFCIP |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MDA-9 antibody MDA9 antibody Melanoma differentiation-associated protein 9 antibody Pro-TGF-alpha cytoplasmic domain-interacting protein 18 antibody Scaffold protein Pbp1 antibody SDCB1_HUMAN antibody SDCBP antibody ST1 antibody SYCL antibody Syndecan binding protein (syntenin) antibody Syndecan binding protein 1 antibody Syndecan-binding protein 1 antibody Syntenin 1 antibody Syntenin-1 antibody TACIP18 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytoplasm. Cytoskeleton. Membrane. Nucleus. Secreted. |
| SwissProt ID | O00560 |
|---|
Application
Fig1: Western blot analysis of Syntenin on A549 cell using anti-Syntenin antibody at 1/2,000 dilution.
Application
Fig2: ICC staining Syntenin in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Syntenin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syntenin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntenin antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with Syntenin antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, SH-SY-5Y, SiHa, human tonsil tissue, human colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
