Application
Fig1: Western blot analysis of TTC11 on SK-Br-3 cell using anti-TTC11 antibody at 1/2,000 dilution.Application
Fig2: ICC staining TTC11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining TTC11 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining TTC11 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TTC11 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TTC11 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TTC11 antibody. Counter stained with hematoxylin.| Product Name | TTC11 [JE40-90] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Fis1 localizes to the outer mitochondrial membrane and, along with dynamin-related protein (Drp1), participates in mitochondrial fission. Fission and fusion mechanisms regulate mitochondrial morphology within the cell. Fission frequency is determined by the level of Fis1 molecules at the mitochondrial surface. Fis1 contains a C-terminal domain, which is required for mitochondrial localization, and an N-terminal domain, which is necessary for mitochondrial fission. Fragmentation of the mitochondrial network by Fis1 leads to cytochrome c release and apoptosis. The mitochondrial fission mechanisms may be involved in positively and negatively regulating apoptosis. |
| Immunogen | Synthetic peptide corresponding to N-terminal Human TTC11. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC,IF,IHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 2010003O14Rik antibody
CGI 135 antibody
CGI135 antibody
FIS 1 antibody
FIS1 antibody
Fis1 homolog antibody
FIS1 S. cerevisiae homolog of antibody FIS1_HUMAN antibody Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody Fission 1 (mitochondrial outer membrane) homolog antibody Fission 1 homolog antibody H NH0132A01.6 antibody hFis 1 antibody hFis1 antibody Mitochondrial fission 1 protein antibody mitochondrial fission molecule antibody Tetratricopeptide repeat domain 11 antibody Tetratricopeptide repeat protein 11 antibody TPR repeat protein 11 antibody TTC 11 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Peroxisome. Mitochondrion. |
| SwissProt ID | Q9Y3D6 |
|---|
Application
Fig1: Western blot analysis of TTC11 on SK-Br-3 cell using anti-TTC11 antibody at 1/2,000 dilution.
Application
Fig2: ICC staining TTC11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TTC11 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining TTC11 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TTC11 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TTC11 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TTC11 antibody. Counter stained with hematoxylin.| Positive Control | SK-Br-3, MCF-7, SH-SY-5Y, human kidney tissue, human small intestine tissue, human uterus tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
