WB
Western blot analysis of PEN2 on different lysates with Rabbit anti-PEN2 antibody at 1/2,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate, Lane 2: 293T (Human embryonic kidney cell) cell lysate, Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate, Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate, Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate, Lane 6: A20 (Mouse B-cell lymphoma cell) cell lysate, Lane 7: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, Lane 8: Mouse spleen tissue lysate, Lane 9: Mouse kidney tissue lysate, Lane 10: Rat lung tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature, Primary antibody:1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.ICC/IF
ICC staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.FC
Flow cytometric analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.IF-F
Immunofluorescence analysis of frozen mouse testis tissue with Rabbit anti-PEN2 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IF-F
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-PEN2 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IF-P
Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling PEN2 with Rabbit anti-PEN2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | PEN2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Four proteins comprise the gamma-secretase complex: presenilin, nicastrin, aph-1, and PEN-2. Together, these proteins mediate cell surface signaling pathways for a variety of type I membrane proteins, notably amyloid-beta precursor protein, a protein implicated in the development of Alzheimer's disease, via intramembrane proteolysis. The proteins assemble into a proteolytically active complex in the golgi/trans-golgi network (TGN) compartments. Assembly leads to autocleavage of presenilin into two subunits to create the active site of gamma-secretase, an important step in understanding the mechanisms involved in the etiology and possible treatment of Alzheimer's disease. |
| Immunogen | Recombinant protein within Human PEN2 aa 1-101 / 101. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIF-FIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 IF-F:1:100 IF-P:1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Gamma secretase subunit PEN 2 antibody Gamma Secretase Subunit PEN2 antibody Gamma-secretase subunit PEN-2 antibody Hematopoietic stem/progenitor cells protein MDS033 antibody MDS033 antibody MSTP064 antibody PEN 2 antibody PEN2_HUMAN antibody Presenilin Enhancer 2 antibody Presenilin enhancer 2 homolog antibody Presenilin enhancer protein 2 antibody PSEN2 antibody psenen antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Cell membrane. Endoplasmic reticulum. Golgi apparatus. |
WB
Western blot analysis of PEN2 on different lysates with Rabbit anti-PEN2 antibody at 1/2,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate, Lane 2: 293T (Human embryonic kidney cell) cell lysate, Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate, Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate, Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate, Lane 6: A20 (Mouse B-cell lymphoma cell) cell lysate, Lane 7: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, Lane 8: Mouse spleen tissue lysate, Lane 9: Mouse kidney tissue lysate, Lane 10: Rat lung tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature, Primary antibody:1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
ICC/IF
ICC staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
FC
Flow cytometric analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
IF-F
Immunofluorescence analysis of frozen mouse testis tissue with Rabbit anti-PEN2 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-F
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-PEN2 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-P
Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling PEN2 with Rabbit anti-PEN2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 IF-F:1:100 IF-P:1:200 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
