WB
Western blot analysis of Brachyury / Bry on 293 cell lysates with Rabbit anti-Brachyury / Bry antibody at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes, Blocking: 5% NFDM/TBST, 1 hour at room temperature, Primary antibody: 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse embryo notochord tissue with Rabbit anti-Brachyury / Bry antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of Brachyury was done on A549 cells. The cells were fixed, permeabilized and stained with Brachyury antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.| Product Name | Brachyury / Bry Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. |
| Immunogen | Recombinant protein within Human Brachyury aa 236-435 / 435. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500 IHC:1:1000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | BRAC_HUMAN antibody
Brachyury homolog antibody
Brachyury protein antibody
Bry antibody
MGC104817 antibody
Protein T antibody
SAVA antibody
T antibody
T brachyury homolog antibody
T brachyury transcription factor antibody
T Protein antibody
T brachyury homolog (mouse) antibody TFT antibody Transcription factor T antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of Brachyury / Bry on 293 cell lysates with Rabbit anti-Brachyury / Bry antibody at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes, Blocking: 5% NFDM/TBST, 1 hour at room temperature, Primary antibody: 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse embryo notochord tissue with Rabbit anti-Brachyury / Bry antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of Brachyury was done on A549 cells. The cells were fixed, permeabilized and stained with Brachyury antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.| Application Notes | WB:1:500 IHC:1:1000 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
