WB
Western blot analysis of RPA32/RPA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate, Lane 2: A431 cell lysate, Lane 3: SH-SY-5Y cell lysate, Lane 4: SiHa cell lysate, Lane 5: 293 cell lysate, Lane 6: Hela cell lysate, Lane 7: HepG2 cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.ICC/IF
Species: Human, Cell sample: HeLa, Antibody concentration: 1: 100.| Product Name | RPA32/RPA2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. |
| Immunogen | Synthetic peptide within Human RPA32 / RPA2 aa 221-270 / 270. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 60S acidic ribosomal protein P1 antibody
AA409079 antibody
AI325195 antibody
AU020965 antibody
ik:tdsubc_2g1 antibody
M(2)21C antibody
MGC137236 antibody
OTTHUMP00000004008 antibody
p32 antibody
p34 antibody
RCJMB04_6d17 replication protein A2 32kDa antibody REPA2 antibody Replication factor A protein 2 antibody Replication protein A 32 kDa subunit antibody Replication protein A 32kDa subunit antibody Replication protein A 34 kDa subunit antibody Replication protein A antibody Replication Protein A2 (32kDa) antibody Replication protein A2 antibody Replication protein A2 32kDa antibody RF-A protein 2 antibody Rf-A2 antibody RFA antibody RFA2_HUMAN antibody RP-A p32 antibody RP-A p34 antibody RP21C antibody RPA 2 antibody RPA 32 antibody RPA antibody Rpa2 antibody RPA32 antibody RPA34 antibody RpLP1 antibody RpP2 antibody xx:tdsubc_2g1 antibody zgc:109822 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of RPA32/RPA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate, Lane 2: A431 cell lysate, Lane 3: SH-SY-5Y cell lysate, Lane 4: SiHa cell lysate, Lane 5: 293 cell lysate, Lane 6: Hela cell lysate, Lane 7: HepG2 cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ICC/IF
Species: Human, Cell sample: HeLa, Antibody concentration: 1: 100.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
