Application
Fig1: Western blot analysis of SERCA1 ATPase on human fetal skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-43) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.| Product Name | SERCA1 ATPase [JE45-75] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | fast twitch skeletal muscle isoform antibody AT2A1_HUMAN antibody ATP2A antibody ATP2A1 antibody ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody ATPase Ca++ transporting fast twitch 1 antibody ATPase, Ca(2+)-transporting fast twitch 1 antibody Calcium pump 1 antibody Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody Calcium-transporting ATPase sarcoplasmic reticulum type antibody EC 3.6.3.8 antibody Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody Fast skeletal muscle SR calcium ATPase antibody OTTHUMP00000162561 antibody OTTHUMP00000162562 antibody Sarcoendoplasmic reticulum calcium ATPase antibody Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody SERCA 1 antibody SERCA1 antibody SERCA1 truncated isoform, included antibody SR Ca(2+) ATPase 1 antibody SR Ca(2+)-ATPase 1 antibody |
| Product description | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. There are 3 major domains on the cytoplasmic face of SERCA: the phosphorylation and nucleotide-binding domains, which form the catalytic site, and the actuator domain, which is involved in the transmission of major conformational changes. It seems that, in addition to the calcium-transporting properties, SERCA1 generates heat in some adipocytes [1][2] and can improve cold tolerance in some wood frogs. |
| Immunogen | Synthetic peptide within human SERCA1 ATPase aa 1-50. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPIHC |
| WB:1:500-1:1,000: IP:1:10-1:50 IHC:1:50-1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | fast twitch skeletal muscle isoform antibody
AT2A1_HUMAN antibody
ATP2A antibody
ATP2A1 antibody
ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
ATPase Ca++ transporting fast twitch 1 antibody
ATPase Ca(2+)-transporting fast twitch 1 antibody Calcium pump 1 antibody Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody Calcium-transporting ATPase sarcoplasmic reticulum type antibody EC 3.6.3.8 antibody Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody Fast skeletal muscle SR calcium ATPase antibody OTTHUMP00000162561 antibody OTTHUMP00000162562 antibody Sarcoendoplasmic reticulum calcium ATPase antibody Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody SERCA 1 antibody SERCA1 antibody SERCA1 truncated isoform included antibody SR Ca(2+) ATPase 1 antibody SR Ca(2+)-ATPase 1 antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane. |
| SwissProt ID | O14983 |
|---|
Application
Fig1: Western blot analysis of SERCA1 ATPase on human fetal skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-43) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.| Positive Control | Human fetal skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000: IP:1:10-1:50 IHC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
