Application
Fig1: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Thyroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-64, 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Thyroglobulin [JE47-61] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AITD 3 antibody AITD3 antibody hTG antibody TDH3 antibody Tg antibody TGN antibody THYG_HUMAN antibody Thyroglobulin antibody |
| Product description | Thyroglobulin (Tg) is a vertebrate secretory protein synthesized in the thyrocyte endoplasmic reticulum (ER), where it acquires N-linked glycosylation and conformational maturation (including formation of many disulfide bonds), leading to homodimerization. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Its primary functions include iodide storage and thyroid hormonogenesis. Tg consists largely of repeating domains, and many tyrosyl residues in these domains become iodinated to form monoiodo- and diiodotyrosine, whereas only a small portion of Tg structure is dedicated to hormone formation. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. |
| Immunogen | Recombinant protein within C-terminal human Thyroglobulin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500: IHC:1:50-1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AITD 3 antibody AITD3 antibody hTG antibody TDH3 antibody Tg antibody TGN antibody THYG_HUMAN antibody Thyroglobulin antibody |
|---|---|
| Molecular Weight(MW) | 305 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P01266 |
|---|
Application
Fig1: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Thyroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-64, 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Human thyroid gland tissue. |
|---|---|
| Application Notes | WB:1:500: IHC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
