Application
Fig1: Western blot analysis of MAD3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: Hela cell lysateApplication
Fig2: Flow cytometric analysis of MAD3 was done on SHG-44 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-76, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | MAD3 [JE48-14] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bHLHc13 antibody Class C basic helix-loop-helix protein 13 antibody FLJ35523 antibody Likely ortholog of mouse Max dimerization protein 3 antibody MAD3_HUMAN antibody MAX dimerization protein 3 antibody Max dimerizer 3 antibody Max-associated protein 3 antibody Max-interacting transcriptional repressor MAD3 antibody MGC2383 antibody mxd3 antibody Myx antibody OTTHUMP00000223596 antibody OTTHUMP00000223598 antibody |
| Product description | MXD3 is a member of the MXD family of basic-helix-loop-helix transcription factors that form heterodimers with the cofactor MAX, and were originally proposed as functional antagonists of MYC proteins in the MYC/MAX/MAD transcriptional network. While some MXD proteins are MYC antagonists by acting as transcriptional repressors to promote cell differentiation, MXD3 is an atypical member that plays a role in proliferation rather than differentiation. MXD3 promotes granule neuron precursor (GNP) proliferation through a Sonic Hedgehog-mediated pathway, it is expressed in a mouse model of medulloblastoma, and its knock-down (KD) reduced proliferation of the human medulloblastoma cell line DAOY. |
| Immunogen | Synthetic peptide within N-terminal human MAD3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | bHLHc13 antibody Class C basic helix-loop-helix protein 13 antibody FLJ35523 antibody Likely ortholog of mouse Max dimerization protein 3 antibody MAD3_HUMAN antibody MAX dimerization protein 3 antibody Max dimerizer 3 antibody Max-associated protein 3 antibody Max-interacting transcriptional repressor MAD3 antibody MGC2383 antibody mxd3 antibody Myx antibody OTTHUMP00000223596 antibody OTTHUMP00000223598 antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of MAD3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: Hela cell lysate
Application
Fig2: Flow cytometric analysis of MAD3 was done on SHG-44 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-76, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | SH-SY5Y, Hela, SHG-44. |
|---|---|
| Application Notes | WB:1:500: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
