Application
Fig1: Western blot analysis of RSK1 p90 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining of RSK1 p90 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig8: Flow cytometric analysis of RSK1 p90 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-83, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | RSK1 p90 [JE48-76] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 90 kDa ribosomal protein S6 kinase 1 antibody dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody dJ590P13.1 antibody EC 2.7.11.1 antibody HU 1 antibody HU1 antibody KS6A1_HUMAN antibody MAP kinase activated protein kinase 1a antibody MAP kinase-activated protein kinase 1a antibody MAPK-activated protein kinase 1a antibody MAPKAP kinase 1a antibody MAPKAPK-1a antibody MAPKAPK1A antibody MGC79981 antibody Mitogen-activated protein kinase-activated protein kinase 1A antibody OTTHUMP00000004113 antibody p90 RSK1 antibody p90-RSK 1 antibody p90rsk antibody p90RSK1 antibody p90S6K antibody pp90RSK1 antibody Ribosomal protein S6 kinase 90kD 1 antibody Ribosomal protein S6 kinase 90kD polypeptide 1 antibody Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody Ribosomal protein S6 kinase alpha 1 antibody Ribosomal protein S6 kinase alpha-1 antibody Ribosomal protein S6 kinase polypeptide 1 antibody Ribosomal S6 kinase 1 antibody RPS6K1 alpha antibody rps6ka antibody Rps6ka1 antibody RSK 1 antibody RSK 1 p90 antibody RSK antibody RSK-1 antibody RSK1 antibody S6K alpha 1 antibody S6K-alpha-1 antibody |
| Product description | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. |
| Immunogen | Recombinant protein within N-terminal human RSK1 p90. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000: ICC:1:50: IHC:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 90 kDa ribosomal protein S6 kinase 1 antibody
dJ590P13.1 (ribosomal protein S6 kinase 90kD polypeptide 1 antibody dJ590P13.1 antibody EC 2.7.11.1 antibody HU 1 antibody HU1 antibody KS6A1_HUMAN antibody MAP kinase activated protein kinase 1a antibody MAP kinase-activated protein kinase 1a antibody MAPK-activated protein kinase 1a antibody MAPKAP kinase 1a antibody MAPKAPK-1a antibody MAPKAPK1A antibody MGC79981 antibody Mitogen-activated protein kinase-activated protein kinase 1A antibody OTTHUMP00000004113 antibody p90 RSK1 antibody p90-RSK 1 antibody p90rsk antibody p90RSK1 antibody p90S6K antibody pp90RSK1 antibody Ribosomal protein S6 kinase 90kD 1 antibody Ribosomal protein S6 kinase 90kD polypeptide 1 antibody Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody Ribosomal protein S6 kinase alpha 1 antibody Ribosomal protein S6 kinase alpha-1 antibody Ribosomal protein S6 kinase polypeptide 1 antibody Ribosomal S6 kinase 1 antibody RPS6K1 alpha antibody rps6ka antibody Rps6ka1 antibody RSK 1 antibody RSK 1 p90 antibody RSK antibody RSK-1 antibody RSK1 antibody S6K alpha 1 antibody S6K-alpha-1 antibody |
|---|---|
| Molecular Weight(MW) | 83 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of RSK1 p90 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining of RSK1 p90 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig8: Flow cytometric analysis of RSK1 p90 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-83, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Daudi cell, Hela cell, rat testis tissue, human appendix tissue, human prostate cancer tissue, human small intestine tissue, mouse colon tissue, F9 cell. |
|---|---|
| Application Notes | WB:1:500-1:2,000: ICC:1:50: IHC:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
