Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | BCL2L12 [JE51-66] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B2L12_HUMAN antibody BCL 2 like 12 (proline rich) antibody BCL 2 like 12 antibody BCL 2 like 12 isoform 1 antibody Bcl 2 like 12 protein antibody Bcl 2 related proline rich protein antibody Bcl-2-like protein 12 antibody Bcl-2-related proline-rich protein antibody BCL2 like 12 (proline rich) antibody BCL2 like 12 antibody BCL2 like 12 isoform 1 antibody Bcl2 like 12 protein antibody Bcl2 related proline rich protein antibody Bcl2-L-12 antibody BCL2L12 antibody BPR antibody MGC120313 antibody MGC120314 antibody MGC120315 antibody |
| Product description | Bcl-2-like protein 12 is a protein that in humans is encoded by the BCL2L12 gene. The protein encoded by this gene belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 2 (BH2). The function of this gene has not yet been determined. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Bcl2L12 expression is upregulated in most human glioblastomas. Expression of Bcl2L12 results in resistance to apoptosis. Bcl2L12 directly neutralizes caspase-7 (CASP7) and indirectly neutralizes caspase-3 (CASP3) by an indirect mechanism.[7] Both caspase enzymes are known to play essential roles in the execution phase of apoptosis. |
| Immunogen | Synthetic peptide within C-terminal human BCL2L12 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHC-PFC |
| WB:1:500-1:2,000: ICC:1:50-1:100: IHC-P:1:200-1:1000: FC:1:50-1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | B2L12_HUMAN antibody
BCL 2 like 12 (proline rich) antibody
BCL 2 like 12 antibody BCL 2 like 12 isoform 1 antibody Bcl 2 like 12 protein antibody Bcl 2 related proline rich protein antibody Bcl-2-like protein 12 antibody Bcl-2-related proline-rich protein antibody BCL2 like 12 (proline rich) antibody BCL2 like 12 antibody BCL2 like 12 isoform 1 antibody Bcl2 like 12 protein antibody Bcl2 related proline rich protein antibody Bcl2-L-12 antibody BCL2L12 antibody BPR antibody MGC120313 antibody MGC120314 antibody MGC120315 antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa (Predicted band size) |
| Cellular Localization | Nucleus, membrane. |
| SwissProt ID | Q9HB09 |
|---|
Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig6: Flow cytometric analysis of BCL2L12 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | MCF-7 cell lysates, PC-3M cell lysates, A431, SW620, human lung carcinoma tissue, human gastric carcinoma tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000: ICC:1:50-1:100: IHC-P:1:200-1:1000: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
