Application
Fig1: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-G9a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-G9a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-G9a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | G9a [JE50-84] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ankyrin repeat containing protein antibody Bat 8 antibody Bat8 antibody C6orf30 antibody DKFZp686H08213 antibody EHMT 2 antibody Ehmt2 antibody EHMT2_HUMAN antibody Euchromatic histone lysine methyltransferase 2 antibody Euchromatic histone lysine N methyltransferase 2 antibody Euchromatic histone-lysine N-methyltransferase 2 antibody FLJ35547 antibody G 9a antibody G9 a antibody G9a protein antibody G9A antibody G9A histone methyltransferase antibody GAT8 antibody H3 K9 HMTase 3 antibody H3-K9-HMTase 3 antibody Histone H3 K9 methyltransferase 3 antibody Histone H3 K9 methyltransferase3 antibody Histone H3-K9 methyltransferase 3 antibody Histone lysine N methyltransferase antibody Histone lysine N methyltransferase EHMT2 antibody Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody Histone-lysine N-methyltransferase EHMT2 antibody HLA B associated transcript 8 antibody HLA-B-associated transcript 8 antibody KMT 1C antibody KMT1 C antibody Lysine N methyltransferase 1C antibody Lysine N-methyltransferase 1C antibody NG 36 antibody NG36 antibody Protein G9a antibody |
| Product description | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| Immunogen | Synthetic peptide within C-terminal human G9A. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500 IHC-P:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Ankyrin repeat containing protein antibody
Bat 8 antibody
Bat8 antibody C6orf30 antibody DKFZp686H08213 antibody EHMT 2 antibody Ehmt2 antibody EHMT2_HUMAN antibody Euchromatic histone lysine methyltransferase 2 antibody Euchromatic histone lysine N methyltransferase 2 antibody Euchromatic histone-lysine N-methyltransferase 2 antibody FLJ35547 antibody G 9a antibody G9 a antibody G9a protein antibody G9A antibody G9A histone methyltransferase antibody GAT8 antibody H3 K9 HMTase 3 antibody H3-K9-HMTase 3 antibody Histone H3 K9 methyltransferase 3 antibody Histone H3 K9 methyltransferase3 antibody Histone H3-K9 methyltransferase 3 antibody Histone lysine N methyltransferase antibody Histone lysine N methyltransferase EHMT2 antibody Histone lysine N methyltransferase H3 lysine 9 specific 3 antibody Histone lysine N methyltransferase H3 lysine 9 specific3 antibody Histone-lysine N-methyltransferase EHMT2 antibody HLA B associated transcript 8 antibody HLA-B-associated transcript 8 antibody KMT 1C antibody KMT1 C antibody Lysine N methyltransferase 1C antibody Lysine N-methyltransferase 1C antibody NG 36 antibody NG36 antibody Protein G9a antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 132 kDa. |
| Cellular Localization | Nucleus, chromosome. |
| SwissProt ID | Q96KQ7 |
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Application
Fig1: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-G9a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-G9a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-G9a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Human colon carcinoma tissue, human breast carcinoma tissue, human gastric carcinoma tissue. |
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| Application Notes | WB:1:500 IHC-P:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
