Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NeuroD2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuroD2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | NeuroD2 [JE50-57] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter (By similarity). |
| Immunogen | Synthetic peptide within C-terminal human NeuroD2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHC-P,ChIP,WB |
| IHC-P:1:50-1:200WB:1:500 |
|
| Species Reactivity | Mouse,Rat,Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | bHLHa1 antibody
class A basic helix loop helix protein 1 antibody
Class A basic helix-loop-helix protein 1 antibody NDF2_HUMAN antibody NDR2 antibody NDRF antibody neuroD related factor antibody NeuroD-related factor antibody NeuroD2 antibody neurogenic basic helix loop helix protein antibody neurogenic differentiation 2 antibody Neurogenic differentiation factor 2 antibody neuronal differentiation 2 antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Nucleus |
Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NeuroD2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuroD2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Rat brain tissue, mouse brain tissue. |
|---|---|
| Application Notes | IHC-P:1:50-1:200WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
