Application
Fig1: Western blot analysis of WRN on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | WRN [JE52-72] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686C2056 antibody DNA helicase antibody DNA helicase, RecQ like type 3 antibody Exonuclease WRN antibody HGNC 12791 antibody OTTHUMP00000225301 antibody RecQ protein-like 2 antibody RecQ-like type 3 antibody RecQ3 antibody RECQL2 antibody RECQL3 antibody Werner syndrome ATP-dependent helicase antibody Werner syndrome helicase antibody Werner syndrome protein antibody Werner syndrome, RecQ helicase like antibody WRN antibody WRN_HUMAN antibody |
| Product description | Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation. |
| Immunogen | Synthetic peptide within human C-terminal WRN. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:1,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp686C2056 antibody
DNA helicase antibody
DNA helicase RecQ like type 3 antibody Exonuclease WRN antibody HGNC 12791 antibody OTTHUMP00000225301 antibody RecQ protein-like 2 antibody RecQ-like type 3 antibody RecQ3 antibody RECQL2 antibody RECQL3 antibody Werner syndrome ATP-dependent helicase antibody Werner syndrome helicase antibody Werner syndrome protein antibody Werner syndrome RecQ helicase like antibody WRN antibody WRN_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 162 kDa |
| Cellular Localization | Nucleolus, nucleus, nucleoplasm. |
| SwissProt ID | Q14191 |
|---|
Application
Fig1: Western blot analysis of WRN on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | K562 cell lysates. |
|---|---|
| Application Notes | WB:1:500-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
