Application
Fig1: Western blot analysis of ERAP1 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | ERAP1 [JE52-36] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A LAP antibody A-LAP antibody Adipocyte derived leucine aminopeptidase antibody Adipocyte-derived leucine aminopeptidase antibody ALAP antibody Aminopeptidase PILS antibody Aminopeptidase regulator of TNFR1 shedding antibody APPILS antibody ARTS 1 antibody ARTS-1 antibody Arts1 antibody Endoplasmic reticulum aminopeptidase 1 antibody Endoplasmic reticulum aminopeptidase associated with antigen processing antibody ERAAP antibody ERAAP1 antibody ERAP 1 antibody Erap1 antibody ERAP1_HUMAN antibody KIAA0525 antibody kiaa0525 protein antibody PILS AP antibody PILS-AP antibody PILSA antibody PILSAP antibody Puromycin insensitive leucyl specific aminopeptidase antibody Puromycin-insensitive leucyl-specific aminopeptidase antibody Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody VEGF-induced aminopeptidase antibody |
| Product description | The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. ERAP1 is also designated adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). Peptides presented by MHC class I on the surface of a cell must be eight to eleven residues long and ERAP1 specifically trims peptides of nine amino acids or more. ERAP1 is induced by interferon-g and encoded for by the ARTS1 gene, which maps to human chromosome 5q15. ERAP1 is thought to inactivate several bioactive peptides, including angioten-sin II, and subsequently, may be involved in the regulation of blood pressure. It may have a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and is characterized as a TNFR1 binding protein that promotes TNFR1 shedding. |
| Immunogen | Synthetic peptide corresponding to C-terminal human ERAP1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:200-1:500: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | A LAP antibody
A-LAP antibody
Adipocyte derived leucine aminopeptidase antibody Adipocyte-derived leucine aminopeptidase antibody ALAP antibody Aminopeptidase PILS antibody Aminopeptidase regulator of TNFR1 shedding antibody APPILS antibody ARTS 1 antibody ARTS-1 antibody Arts1 antibody Endoplasmic reticulum aminopeptidase 1 antibody Endoplasmic reticulum aminopeptidase associated with antigen processing antibody ERAAP antibody ERAAP1 antibody ERAP 1 antibody Erap1 antibody ERAP1_HUMAN antibody KIAA0525 antibody kiaa0525 protein antibody PILS AP antibody PILS-AP antibody PILSA antibody PILSAP antibody Puromycin insensitive leucyl specific aminopeptidase antibody Puromycin-insensitive leucyl-specific aminopeptidase antibody Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody VEGF-induced aminopeptidase antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. |
| SwissProt ID | Q9NZ08 |
|---|
Application
Fig1: Western blot analysis of ERAP1 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Human skin tissue lysates. |
|---|---|
| Application Notes | WB:1:200-1:500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
