Application
Fig1: Western blot analysis of DBF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lane 3: SW480 cell lysate Lane 4: Jurkat cell lysateApplication
Fig2: Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | DBF4 [JE52-50] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activator of S phase kinase antibody ASK antibody CHIF antibody Chiffon homolog A antibody DBF4 antibody DBF4 homolog (S. cerevisiae) antibody DBF4 homolog antibody DBF4 type zinc finger containing protein 1 antibody DBF4 zinc finger A antibody DBF4 zinc finger antibody DBF4, S. cerevisiae, homolog of antibody DBF4-type zinc finger-containing protein 1 antibody DBF4A antibody DBF4A_HUMAN antibody Dumbbell former 4, S. cerevisiae, homolog of antibody OTTHUMP00000161661 antibody Protein DBF4 homolog A antibody ZDBF1 antibody Zinc finger, DBF-type containing 1 antibody |
| Product description | The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire. |
| Immunogen | Synthetic peptide within N-terminal human DBF4. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Activator of S phase kinase antibody
ASK antibody
CHIF antibody Chiffon homolog A antibody DBF4 antibody DBF4 homolog (S. cerevisiae) antibody DBF4 homolog antibody DBF4 type zinc finger containing protein 1 antibody DBF4 zinc finger A antibody DBF4 zinc finger antibody DBF4 S. cerevisiae homolog of antibody DBF4-type zinc finger-containing protein 1 antibody DBF4A antibody DBF4A_HUMAN antibody Dumbbell former 4 S. cerevisiae homolog of antibody OTTHUMP00000161661 antibody Protein DBF4 homolog A antibody ZDBF1 antibody Zinc finger DBF-type containing 1 antibody |
|---|---|
| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9UBU7 |
|---|
Application
Fig1: Western blot analysis of DBF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lane 3: SW480 cell lysate Lane 4: Jurkat cell lysate
Application
Fig2: Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | 293T cell lysate, Hela cell lysate, SW480 cell lysate, Jurkat cell lysate, F9. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
