Application
Fig1: Western blot analysis of Elongation factor 1-gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: K562 cell lysateApplication
Fig2: Western blot analysis of Elongation factor 1-gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse thymus tissue lysate Lane 2: rat spleen tissue lysate Lane 3: mouse ovary tissue lysate Lane 4: rat thymus tissue lysateApplication
Fig3: ICC staining of Elongation factor 1-gamma in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-45, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig4: ICC staining of Elongation factor 1-gamma in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig5: ICC staining of Elongation factor 1-gamma in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig10: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig11: Flow cytometric analysis of Elongation factor 1-gamma was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-45, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Elongation factor 1-gamma [JE54-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2610301D06Rik antibody AA407312 antibody eEF 1B gamma antibody EEF 1G antibody eEF-1B gamma antibody EEF1G antibody EF 1 gamma antibody EF 1G antibody EF-1-gamma antibody EF1 gamma antibody EF1G antibody EF1G_HUMAN antibody Elongation factor 1 gamma antibody Elongation factor 1-gamma antibody Eukaryotic translation elongation factor 1 gamma antibody GIG 35 antibody GIG35 antibody MGC103354 antibody MGC114210 antibody MGC144723 antibody MGC144724 antibody MGC94929 antibody Pancreatic tumor related protein antibody PRO1608 antibody Translation elongation factor eEF 1 gamma chain antibody |
| Product description | N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. EF-1 γ (elongation factor 1-gamma), also known as EEF1G or GIG35, is a 437 amino acid subunit of the EF-1 complex. Expressed in stomach, pancreas, brain, lung, kidney, intestine, liver and spleen, EF-1 γ contains an N-terminal glutathione transferase domain which is thought to be involved in anchoring the complex to various cellular components. Additionally, EF-1 γ may play a key role in the assembly of multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 γ is associated with pancreatic cancer, suggesting a possible role for EF-1 γ in the oncogenic transformation process. |
| Immunogen | Synthetic peptide within N terminal human Elongation factor 1-gamma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHC-PFC |
| WB:1:500-1:2,000: ICC:1:50-1:100: IHC-P:1:200-1:1000: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 2610301D06Rik antibody
AA407312 antibody
eEF 1B gamma antibody EEF 1G antibody eEF-1B gamma antibody EEF1G antibody EF 1 gamma antibody EF 1G antibody EF-1-gamma antibody EF1 gamma antibody EF1G antibody EF1G_HUMAN antibody Elongation factor 1 gamma antibody Elongation factor 1-gamma antibody Eukaryotic translation elongation factor 1 gamma antibody GIG 35 antibody GIG35 antibody MGC103354 antibody MGC114210 antibody MGC144723 antibody MGC144724 antibody MGC94929 antibody Pancreatic tumor related protein antibody PRO1608 antibody Translation elongation factor eEF 1 gamma chain antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytosol, extracellular exosome, nucleus, cytoplasm, membrane. |
Application
Fig1: Western blot analysis of Elongation factor 1-gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: K562 cell lysate
Application
Fig2: Western blot analysis of Elongation factor 1-gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse thymus tissue lysate Lane 2: rat spleen tissue lysate Lane 3: mouse ovary tissue lysate Lane 4: rat thymus tissue lysate
Application
Fig3: ICC staining of Elongation factor 1-gamma in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-45, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig4: ICC staining of Elongation factor 1-gamma in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig5: ICC staining of Elongation factor 1-gamma in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-45, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig11: Flow cytometric analysis of Elongation factor 1-gamma was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-45, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | MCF-7 cell lysate, K562 cell lysate, mouse thymus tissue lysate, rat spleen tissue lysate, mouse ovary tissue lysate, rat thymus tissue lysate, PANC-1, SKOV-3, human colon carcinoma tissue, human small intestine tissue, human pancreas tissue, mouse brain tissue, rat cerebellum tissue, Daudi. |
|---|---|
| Application Notes | WB:1:500-1:2,000: ICC:1:50-1:100: IHC-P:1:200-1:1000: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
