Application
Fig1: Western blot analysis of CD1a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skin tissue lysate Lane 2: Human thymus tissue lysateApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | CD1a [JE28-74] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 1a antibody CD1 antibody CD1a antibody CD1A Antigen antibody CD1A antigen, a polypeptide antibody CD1a molecule antibody CD1A_HUMAN antibody cluster of differentiation 1 A antibody cortical thymocyte antigen CD1A antibody differentiation antigen CD1 alpha 3 antibody epidermal dendritic cell marker CD1a antibody FCB 6 antibody FCB6 antibody HTA 1 antibody HTA1 antibody hTa1 thymocyte antigen antibody OTTHUMP00000018907 antibody R 4 antibody R4 antibody T 6 antibody T-cell surface antigen T6/Leu-6 antibody T-cell surface glycoprotein CD1a antibody T6 antibody |
| Product description | This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined. |
| Immunogen | Synthetic peptide corresponding to N terminal human CD1a. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-1:2,000: IHC-P:1:50-1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 1a antibody
CD1 antibody
CD1a antibody CD1A Antigen antibody CD1A antigen a polypeptide antibody CD1a molecule antibody CD1A_HUMAN antibody cluster of differentiation 1 A antibody cortical thymocyte antigen CD1A antibody differentiation antigen CD1 alpha 3 antibody epidermal dendritic cell marker CD1a antibody FCB 6 antibody FCB6 antibody HTA 1 antibody HTA1 antibody hTa1 thymocyte antigen antibody OTTHUMP00000018907 antibody R 4 antibody R4 antibody T 6 antibody T-cell surface antigen T6/Leu-6 antibody T-cell surface glycoprotein CD1a antibody T6 antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 37 kDa. |
| Cellular Localization | Endosome membrane, cell membrane, membrane raft. |
| SwissProt ID | P06126 |
|---|
Application
Fig1: Western blot analysis of CD1a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skin tissue lysate Lane 2: Human thymus tissue lysate
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Human skin tissue lysate, human thymus tissue lysate, human skin tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000: IHC-P:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
