WB
Western blot analysis of Cytokeratin 8 on different lysates with Mouse anti-Cytokeratin 8 antibody at 1/2,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 24 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Mouse anti-Cytokeratin 8 antibody, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureICC/IF
Immunocytochemical staining of MCF-7 cells using anti-Cytokeratin 8 Mouse mAb.ICC/IF
Immunocytochemical staining of PANC-1 cells using anti-Cytokeratin 8 Mouse mAb.IHC
Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-Cytokeratin 8 Mouse mAb.IHC
Immunohistochemical analysis of paraffin- embedded mouse small intestine tissue using anti-Cytokeratin 8 Mouse mAb.| Product Name | Cytokeratin 8 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy. |
| Immunogen | Synthetic peptide within C-terminal human Cytokeratin 8 aa 441-483. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT8 |
|---|---|
| Gene Synonyms | K8 KO CK8 CK-8 CYK8 K2C8 CARD2 |
| Gene Full Name | keratin 8 |
| Gene Summary | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Molecular Weight(MW) | 53.7 kDa(Observed band size: 40-54 kDa) |
| Cellular Localization | Cytoplasm, Nucleus, Nucleus matrix. |
WB
Western blot analysis of Cytokeratin 8 on different lysates with Mouse anti-Cytokeratin 8 antibody at 1/2,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 24 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Mouse anti-Cytokeratin 8 antibody, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
ICC/IF
Immunocytochemical staining of MCF-7 cells using anti-Cytokeratin 8 Mouse mAb.
ICC/IF
Immunocytochemical staining of PANC-1 cells using anti-Cytokeratin 8 Mouse mAb.
IHC
Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-Cytokeratin 8 Mouse mAb.
IHC
Immunohistochemical analysis of paraffin- embedded mouse small intestine tissue using anti-Cytokeratin 8 Mouse mAb.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
