WB
Western blot analysis on A：HepG2 B：NIH/3T3 C：Rat liver cell lysates using anti-Histone H3 polyclonal antibody.| Product Name | Histone H3 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. This Antibody detects endogenous levels of total histone H3 protein, and does not cross-react with other histones. |
| Immunogen | Synthetic peptide within human Histone H3 aa 1-50. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | HIST1 cluster H3J antibody Histone gene cluster 1 H3 histone family member E antibody Histone gene cluster 1 H3G antibody H3 histone family member J antibody HIST1 cluster H3E antibody HIST1 cluster H3I antibody Histone gene cluster 1 H3C antibody FLJ92264 antibody H3 histone family member A antibody H3 histone family member B antibody H3 histone family member C antibody H3 histone family member D antibody H3 histone family member F antibody H3 histone family member H antibody H3 histone family member I antibody H3 histone family member K antibody H3 histone family member L antibody H3 histone family member T antibody H3 histone family 3A antibody H3.1 antibody H3.3A antibody H3/a antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/l antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3 antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FF antibody H3FH antibody H3FI antibody H3FJ antibody H3FK antibody H3FL antibody HIST1 cluster H3A antibody HIST1 cluster H3B antibody HIST1 cluster H3C antibody HIST1 cluster H3D antibody HIST1 cluster H3F antibody HIST1 cluster H3G antibody HIST1 cluster H3H antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1 H3a antibody Histone 1 H3b antibody Histone 1 H3c antibody Histone 1 H3d antibody Histone 1 H3e antibody Histone 1 H3f antibody Histone 1 H3g antibody Histone 1 H3h antibody Histone 1 H3i antibody Histone 1 H3j antibody histone 3 H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1 H3a antibody Histone cluster 1 H3b antibody Histone cluster 1 H3c antibody Histone cluster 1 H3d antibody Histone cluster 1 H3e antibody Histone cluster 1 H3f antibody Histone cluster 1 H3g antibody Histone cluster 1 H3i antibody Histone cluster 1 H3j antibody Histone gene cluster 1 H3 histone family member A antibody Histone gene cluster 1 H3 histone family member B antibody Histone gene cluster 1 H3 histone family member C antibody Histone gene cluster 1 H3 histone family member D antibody Histone gene cluster 1 H3 histone family member F antibody Histone gene cluster 1 H3 histone family member G antibody Histone gene cluster 1 H3 histone family member H antibody Histone gene cluster 1 H3 histone family member I antibody Histone gene cluster 1 H3 histone family member J antibody Histone gene cluster 1 H3A antibody Histone gene cluster 1 H3B antibody Histone gene cluster 1 H3D antibody Histone gene cluster 1 H3E antibody Histone gene cluster 1 H3F antibody Histone gene cluster 1 H3H antibody Histone gene cluster 1 H3I antibody Histone gene cluster 1 H3J antibody Histone H 3 antibody Histone H3.1 antibody histone H3.1t antibody Histone H3.2 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody Histone H4/o antibody |
|---|---|
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cell nucleus |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis on A：HepG2 B：NIH/3T3 C：Rat liver cell lysates using anti-Histone H3 polyclonal antibody.| Application Notes | WB:1:1000-1:2000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
