Application
Fig1: Western blot analysis of CD73 on different cell lysates using anti-CD73 antibody at 1/1,000 dilution.Positive control：Lane1: HepG2Lane2: HT-29Application
Fig2: ICC staining CD73 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD73 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Jurkat cells with CD73 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CD73 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5' NT antibody 5' nucleotidase (CD73) antibody 5' nucleotidase precursor antibody 5' nucleotidase, ecto antibody 5' nucleotidase, ecto (CD73) antibody 5'-NT antibody 5'-nucleotidase antibody 5NTD_HUMAN antibody CD73 antibody CD73 antigen antibody E5NT antibody Ecto 5' nucleotidase antibody Ecto-5'-nucleotidase antibody eN antibody eNT antibody NT antibody NT5 antibody NT5E antibody NTE antibody Purine 5 Prime Nucleotidase antibody |
| Product description | CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of human CD73 (aa 240-270). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 5' NT antibody
5' nucleotidase (CD73) antibody
5' nucleotidase precursor antibody
5' nucleotidase ecto antibody 5' nucleotidase ecto (CD73) antibody 5'-NT antibody 5'-nucleotidase antibody 5NTD_HUMAN antibody CD73 antibody CD73 antigen antibody E5NT antibody Ecto 5' nucleotidase antibody Ecto-5'-nucleotidase antibody eN antibody eNT antibody NT antibody NT5 antibody NT5E antibody NTE antibody Purine 5 Prime Nucleotidase antibody |
|---|---|
| Molecular Weight(MW) | 63kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of CD73 on different cell lysates using anti-CD73 antibody at 1/1,000 dilution.Positive control：Lane1: HepG2Lane2: HT-29
Application
Fig2: ICC staining CD73 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD73 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Jurkat cells with CD73 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Hela, A549, Jurkat, HT-29, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
