Application
Fig1: Western blot analysis of extracts from cells expressing C-terminal His-tagged protein (lane 1) or N-terminal His-tagged protein (lane 2) using 6X His-tag antibody.| Product Name | 6X His-Tag |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6 His epitope tag antibody Hexa His tag antibody HHHHHH epitope tag antibody HHHHHH tag antibody His tag antibody Polyhistidine Tag antibody |
| Product description | His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2,000-1:5,000 |
|
| Concentration | 1mg/ml |
| Alternative Names | 6 His epitope tag antibody Hexa His tag antibody HHHHHH epitope tag antibody HHHHHH tag antibody His tag antibody Polyhistidine Tag antibody |
|---|
Application
Fig1: Western blot analysis of extracts from cells expressing C-terminal His-tagged protein (lane 1) or N-terminal His-tagged protein (lane 2) using 6X His-tag antibody.| Positive Control | C-terminal or N-terminal His-tagged recombinant protein |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
