WB
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody at 1/20,000 dilution. Lane 1: 293T cell lysate, Lane 2: 293T transfected with Myc-tagged Claudin18.2 (C-terminal) cell lysate, Lane 3: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of 293T cells labeling Myc tag with Rabbit anti-Myc tag antibody at 1/200 dilution. 293T cells, transfected with Myc-tagged empty control, Claudin18.2 (C-terminal) or Histone H3.1 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myc tag antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Myc Tag (green) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of 293T cells transfected C-myc-tag labeling Myc tag. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with Rabbit anti-Myc tag antibody at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using Myc tag Mouse Monoclonal Antibody at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: Rabbit anti-Myc tag antibody IP in Myc Tag fusion protein lysate. Lane 3: Rabbit IgG instead of Rabbit anti-Myc tag antibody in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.| Product Name | Myc tag Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects endogenous levels of total c-Myc protein and is also for detection of Myc-tagged fusion proteins. |
| Immunogen | Synthetic peptide within human Myc aa 410-420. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:5000-1:20000 ICC/IF:1:200 FC:1:1000 IP:2-5 µg/ml. |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c-Myc antibody class E basic helix-loop-helix protein 39 antibody MRTL antibody MYC antibody Myc Epitope Tag antibody myc proto-oncogene protein antibody myc-related translation/localization regulatory factor antibody oncogene c-Myc antibody proto-oncogene c-Myc antibody protooncogene homologous to myelocytomatosis virus antibody transcription factor p64 antibody v-myc avian myelocytomatosis viral oncogene homolog antibody v-myc myelocytomatosis viral oncogene homolog (avian) antibody |
|---|
| SwissProt ID | P01106 |
|---|
WB
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody at 1/20,000 dilution. Lane 1: 293T cell lysate, Lane 2: 293T transfected with Myc-tagged Claudin18.2 (C-terminal) cell lysate, Lane 3: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of 293T cells labeling Myc tag with Rabbit anti-Myc tag antibody at 1/200 dilution. 293T cells, transfected with Myc-tagged empty control, Claudin18.2 (C-terminal) or Histone H3.1 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myc tag antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Myc Tag (green) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of 293T cells transfected C-myc-tag labeling Myc tag. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with Rabbit anti-Myc tag antibody at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using Myc tag Mouse Monoclonal Antibody at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: Rabbit anti-Myc tag antibody IP in Myc Tag fusion protein lysate. Lane 3: Rabbit IgG instead of Rabbit anti-Myc tag antibody in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.| Application Notes | WB:1:5000-1:20000 ICC/IF:1:200 FC:1:1000 IP:2-5 µg/ml. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
