Application
Fig1: Western blot analysis of TRIM28 on different cell lysates using anti-TRIM28 antibody at 1/1,000 dilution.Application
Fig2: ICC staining TRIM28 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with TRIM28 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | TRIM28 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 SUMO protein ligase TRIM28 antibody E3 SUMO-protein ligase TRIM28 antibody FLJ29029 antibody KAP 1 antibody KAP-1 antibody KRAB associated protein 1 antibody KRAB interacting protein 1 antibody KRAB-associated protein 1 antibody KRAB-interacting protein 1 antibody KRIP 1 antibody KRIP-1 antibody KRIP1 antibody Nuclear corepressor KAP 1 antibody Nuclear corepressor KAP-1 antibody RING finger protein 96 antibody RNF96 antibody TF1B antibody TIF1 beta antibody TIF1-beta antibody TIF1B antibody TIF1B_HUMAN antibody Transcription intermediary factor 1 beta antibody Transcription intermediary factor 1-beta antibody Trim28 antibody Tripartite motif containing 28 antibody tripartite motif containing protein 28 antibody Tripartite motif-containing protein 28 antibody |
| Product description | TRIM28, also known as transcriptional intermediary factor 1β (TIF1β) and KAP1, is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TIF1-beta mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. |
| Immunogen | Synthetic peptide within human TRIM28 400-500 aa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | E3 SUMO protein ligase TRIM28 antibody E3 SUMO-protein ligase TRIM28 antibody FLJ29029 antibody KAP 1 antibody KAP-1 antibody KRAB associated protein 1 antibody KRAB interacting protein 1 antibody KRAB-associated protein 1 antibody KRAB-interacting protein 1 antibody KRIP 1 antibody KRIP-1 antibody KRIP1 antibody Nuclear corepressor KAP 1 antibody Nuclear corepressor KAP-1 antibody RING finger protein 96 antibody RNF96 antibody TF1B antibody TIF1 beta antibody TIF1-beta antibody TIF1B antibody TIF1B_HUMAN antibody Transcription intermediary factor 1 beta antibody Transcription intermediary factor 1-beta antibody Trim28 antibody Tripartite motif containing 28 antibody tripartite motif containing protein 28 antibody Tripartite motif-containing protein 28 antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Nucleus |
Application
Fig1: Western blot analysis of TRIM28 on different cell lysates using anti-TRIM28 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining TRIM28 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with TRIM28 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Hela, NCCIT, F9, PC-3M, MCF-7, A431, human liver cancer tissue, human spleen tissue, human breast cancer tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
