Application
Fig1: Western blot analysis on different cell lysates using anti-nucleolin rabbit polyclonal antibodies.Application
Fig2: ICC staining nucleolin in mouse embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).| Product Name | Nucleolin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C23 antibody FLJ45706 antibody MS1116 antibody NCL antibody Nucl antibody NUCL_HUMAN antibody Nucleolin antibody Protein C23 antibody |
| Product description | Nucleolin is a multi-functional protein that is one of the major components of the nucleoli. It plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus. While the main function of nucleolin is ribosome biogenesis, it plays an important role in various other nuclear activities. Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC |
| WB:1:1,000 ICC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | C23 antibody FLJ45706 antibody MS1116 antibody NCL antibody Nucl antibody NUCL_HUMAN antibody Nucleolin antibody Protein C23 antibody |
|---|---|
| Molecular Weight(MW) | ~100kDa |
| Cellular Localization | Cell nucleus, cytoplasm |
| SwissProt ID | P19338 |
|---|
Application
Fig1: Western blot analysis on different cell lysates using anti-nucleolin rabbit polyclonal antibodies.
Application
Fig2: ICC staining nucleolin in mouse embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).| Positive Control | K562, Hela, Jurkat, 293T, mouse embryonic stem cell |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
