Application
Fig1: Western blot analysis on different cell lysates using anti-nucleolin rabbit polyclonal antibodies.Application
Fig2： Immunofluorescent staining of Hela cells using anti- nucleolin rabbit polyclonal antibody.Application
Fig3：Immunohistochemical analysis of paraffin-embedded human ovary tissue using anti-nucleolin rabbit polyclonal antibody.| Product Name | Nucleolin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C23 antibody FLJ45706 antibody MS1116 antibody NCL antibody Nucl antibody NUCL_HUMAN antibody Nucleolin antibody Protein C24 antibody |
| Product description | Nucleolin is a multi-functional protein that is one of the major components of the nucleoli. It plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus. While the main function of nucleolin is ribosome biogenesis, it plays an important role in various other nuclear activities. Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:1,000 ICC:1:50-1:100 IHC:1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | C23 antibody FLJ45706 antibody MS1116 antibody NCL antibody Nucl antibody NUCL_HUMAN antibody Nucleolin antibody Protein C24 antibody |
|---|---|
| Molecular Weight(MW) | ~100kDa |
| Cellular Localization | Cell nucleus, cytoplasm |
| SwissProt ID | P19338 |
|---|
Application
Fig1: Western blot analysis on different cell lysates using anti-nucleolin rabbit polyclonal antibodies.
Application
Fig2： Immunofluorescent staining of Hela cells using anti- nucleolin rabbit polyclonal antibody.
Application
Fig3：Immunohistochemical analysis of paraffin-embedded human ovary tissue using anti-nucleolin rabbit polyclonal antibody.| Positive Control | K562, Hela, human ovary, Jurkat, 293T |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:100 IHC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
