Application
Fig1: Western blot analysis on 293 cell lysates using anti-Cyclin B1 rabbit polyclonal antibodies.Application
Fig2: Immunofluorescent staining of F9 cells using anti- Cyclin B1 rabbit polyclonal antibody.Application
Fig3: Immunofluorescent staining of Hela cells using anti- Cyclin B1 rabbit polyclonal antibody.| Product Name | Cyclin B1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCNB 1 antibody CCNB antibody ccnb1 antibody CCNB1_HUMAN antibody Cyclin B1 antibody G2 mitotic specific cyclin B1 antibody G2/mitotic-specific cyclin-B1 antibody |
| Product description | Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CCNB 1 antibody CCNB antibody ccnb1 antibody CCNB1_HUMAN antibody Cyclin B1 antibody G2 mitotic specific cyclin B1 antibody G2/mitotic-specific cyclin-B1 antibody |
|---|---|
| Molecular Weight(MW) | ~58kDa |
| Cellular Localization | Cytoplasm, nucleus |
| SwissProt ID | P14635 |
|---|
Application
Fig1: Western blot analysis on 293 cell lysates using anti-Cyclin B1 rabbit polyclonal antibodies.
Application
Fig2: Immunofluorescent staining of F9 cells using anti- Cyclin B1 rabbit polyclonal antibody.
Application
Fig3: Immunofluorescent staining of Hela cells using anti- Cyclin B1 rabbit polyclonal antibody.| Positive Control | 293, Hela, F9 |
|---|---|
| Application Notes | IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
