Application
Fig1: Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining TESPA1 in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with TESPA1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | TESPA1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPC257 antibody K0748_HUMAN antibody KIAA0748 antibody Protein TESPA1 antibody TESPA1 – thymocyte expressed, positive selection associated 1 antibody TESPA1 antibody thymocyte expressed, positive selection associated 1 antibody Uncharacterized protein KIAA0748 antibody |
| Product description | TESPA1 (Thymocyte-expressed positive selection-associated protein 1) is required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development. It plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes. |
| Immunogen | Synthetic peptide (KLH-coupled) corresponding to the aa 210-250 of human TESPA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HSPC257 antibody
K0748_HUMAN antibody
KIAA0748 antibody
Protein TESPA1 antibody
TESPA1 – thymocyte expressed positive selection associated 1 antibody TESPA1 antibody thymocyte expressed positive selection associated 1 antibody Uncharacterized protein KIAA0748 antibody |
|---|---|
| Molecular Weight(MW) | 50kDa |
| Cellular Localization | Cytoplasm. Membrane. |
Application
Fig1: Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining TESPA1 in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with TESPA1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Human thymus, mouse thymus, human liver, A431, A172, rat brain, human lung cancer, mouse brain, mouse pancreas, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
