Application
Fig1: Western blot analysis of CDK2 on different cell lysates using anti-CDK2 antibody at 1/1,000 dilution.Positive control:Lane1: A549 Lane2: F9Lane3: PC-12 Lane4: Mouse thymusLane5: Human liverApplication
Fig2: ICC staining CDK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDK2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDK2 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with CDK2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CDK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdc2 related protein kinase antibody cdc2-related protein kinase antibody CDC28 antibody CDC2A antibody Cdk 2 antibody CDK1 antibody CDK2 antibody CDK2_HUMAN antibody CDKN2 antibody Cell devision kinase 2 antibody Cell division protein kinase 2 antibody Cyclin dependent kinase 2 antibody cyclin dependent kinase 2-alpha antibody Cyclin-dependent kinase 2 antibody kinase Cdc2 antibody MPF antibody p33 protein kinase antibody p33(CDK2) antibody |
| Product description | Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1. |
| Immunogen | Synthetic peptide within C terminal residues of human CDK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cdc2 related protein kinase antibody cdc2-related protein kinase antibody CDC28 antibody CDC2A antibody Cdk 2 antibody CDK1 antibody CDK2 antibody CDK2_HUMAN antibody CDKN2 antibody Cell devision kinase 2 antibody Cell division protein kinase 2 antibody Cyclin dependent kinase 2 antibody cyclin dependent kinase 2-alpha antibody Cyclin-dependent kinase 2 antibody kinase Cdc2 antibody MPF antibody p33 protein kinase antibody p33(CDK2) antibody |
|---|---|
| Molecular Weight(MW) | ~33kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of CDK2 on different cell lysates using anti-CDK2 antibody at 1/1,000 dilution.Positive control:Lane1: A549 Lane2: F9Lane3: PC-12 Lane4: Mouse thymusLane5: Human liver
Application
Fig2: ICC staining CDK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDK2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDK2 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with CDK2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, F9, PC-12, mouse thymus tissue, human liver tissue, human tonsil tissue, human breast cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
