Application
Fig1: Western blot analysis on Hela cell lysates using anti-CHFR rabbit polyclonal antibodies.Application
Fig2: ICC staining CHFR in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).| Product Name | CHFR |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Checkpoint with forkhead and ring finger domains antibody Checkpoint with forkhead and RING finger domains protein antibody Chfr antibody CHFR_HUMAN antibody E3 ubiquitin protein ligase CHFR antibody E3 ubiquitin-protein ligase CHFR antibody EC 6.3.2. antibody FLJ10796 antibody FLJ33629 antibody RING finger protein 196 antibody RNF 116 antibody RNF 196 antibody RNF116 antibody RNF196 antibody Ubiquitin ligase protein antibody |
| Product description | E3 ubiquitin-protein ligase (CHFR) that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. CHFR is probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation. Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC |
| WB:1:500-1:1,000 ICC:1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Checkpoint with forkhead and ring finger domains antibody Checkpoint with forkhead and RING finger domains protein antibody Chfr antibody CHFR_HUMAN antibody E3 ubiquitin protein ligase CHFR antibody E3 ubiquitin-protein ligase CHFR antibody EC 6.3.2. antibody FLJ10796 antibody FLJ33629 antibody RING finger protein 196 antibody RNF 116 antibody RNF 196 antibody RNF116 antibody RNF196 antibody Ubiquitin ligase protein antibody |
|---|---|
| Molecular Weight(MW) | ~73kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q96EP1 |
|---|
Application
Fig1: Western blot analysis on Hela cell lysates using anti-CHFR rabbit polyclonal antibodies.
Application
Fig2: ICC staining CHFR in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).| Positive Control | Hela, A431 |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
