Application
Fig1: Western blot analysis on cell lysates using anti-APAF1 rabbit polyclonal antibodies.| Product Name | APAF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APAF 1 antibody Apaf-1 antibody APAF_HUMAN antibody Apaf1 antibody Apoptotic peptidase activating factor 1 antibody Apoptotic protease activating factor 1 antibody Apoptotic protease activating factor antibody Apoptotic protease-activating factor 1 antibody CED 4 antibody CED4 antibody KIAA0413 antibody |
| Product description | Apoptotic protease activating factor 1, also known as APAF1, is a human homolog of C. elegans CED-4 gene. This gene encodes a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. This protein contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. The precise mechanism for this reaction is still debated though work published by Guy Salvesen suggests that the apoptosome may induce caspase 9 dimerization and subsequent autocatalysis. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | APAF 1 antibody Apaf-1 antibody APAF_HUMAN antibody Apaf1 antibody Apoptotic peptidase activating factor 1 antibody Apoptotic protease activating factor 1 antibody Apoptotic protease activating factor antibody Apoptotic protease-activating factor 1 antibody CED 4 antibody CED4 antibody KIAA0413 antibody |
|---|---|
| Molecular Weight(MW) | 142 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | O14727 |
|---|
Application
Fig1: Western blot analysis on cell lysates using anti-APAF1 rabbit polyclonal antibodies.| Positive Control | HepG2, THP-1 |
|---|---|
| Application Notes | WB:1:500-1:1000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
