Application
Fig1: Western blot analysis on rat kidney lysates using anti-ATG12 rabbit polyclonal antibodies.Application
Fig2: ICC staining ATG12 in HCT116 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | ATG12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APG12-like antibody APG12L antibody ATG12 antibody ATG12 autophagy related 12 homolog (S. cerevisiae) antibody ATG12 autophagy related 12 homolog antibody ATG12_HUMAN antibody Autophagy 12 antibody Autophagy-related protein 12 antibody FBR93 antibody HAPG12 antibody Ubiquitin-like protein ATG12 antibody |
| Product description | Autophagy-related protein 12 is a protein that in humans is encoded by the ATG12 gene. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. Autophagy requires the covalent attachment of the protein Atg12 to ATG5 through a ubiquitin-like conjugation system. The Atg12-Atg5 conjugate then promotes the conjugation of ATG8 to the lipid phosphatidylethanolamine. Atg12 was found to be involved in apoptosis. This protein promotes apoptosis through an interaction with anti-apoptotic members of the Bcl-2 family. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1000 ICC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | APG12-like antibody APG12L antibody ATG12 antibody ATG12 autophagy related 12 homolog (S. cerevisiae) antibody ATG12 autophagy related 12 homolog antibody ATG12_HUMAN antibody Autophagy 12 antibody Autophagy-related protein 12 antibody FBR93 antibody HAPG12 antibody Ubiquitin-like protein ATG12 antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | O94817 |
|---|
Application
Fig1: Western blot analysis on rat kidney lysates using anti-ATG12 rabbit polyclonal antibodies.
Application
Fig2: ICC staining ATG12 in HCT116 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Rat kidney, HCT116 |
|---|---|
| Application Notes | WB:1:500-1:1000 ICC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
