Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.
Immunogen
This antibody is produced by immunizing rabbits with a synthetic peptide GLNDIFEAQKIEWHE (KLH-coupled).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WBICC/IF
WB:1:10,000 ELISA:1:10,000
Concentration
1mg/ml
Application
Application
Fig1: Western blot analysis on Avi tagged fusion protein.
Positive Control
Avi- tagged fusion protein
Application Notes
WB:1:10,000 ELISA:1:10,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.