Application
Fig1: Western blot analysis on purified restriction enzyme FokI.| Product Name | Restriction enzyme FokI(C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 66kDa |
|---|
| SwissProt ID | P14870 |
|---|
Application
Fig1: Western blot analysis on purified restriction enzyme FokI.| Positive Control | Purified restriction enzyme FokI |
|---|---|
| Application Notes | WB:1:1000-1:2000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
